1.
Methods Mol Biol
; 2311: 185-193, 2021.
Article
in English
| MEDLINE | ID: covidwho-1482181
ABSTRACT
Studies of blood-brain barrier (BBB) require developing of a novel and convenient in vitro endothelial cell model. We isolated primary human and rodent brain microvascular endothelial cells and developed methods for culturing, characterization, and high-efficiency transfection of endothelial cells. Here, we describe the improved methods to obtain in vitro human and rodent BBB models to study expression of endogenous and exogenous genes of interest.